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Image Search Results
Journal: Frontiers in Molecular Biosciences
Article Title: miR-124-3p Suppresses the Invasiveness and Metastasis of Hepatocarcinoma Cells via Targeting CRKL
doi: 10.3389/fmolb.2020.00223
Figure Lengend Snippet: (A) The expression level changes of proteins RAF 0.363 ± 0.015 ( P = 0.0001), MEK 0.220 ± 0.026 ( P = 0.0001), ERK1/2 0.506 ± 0.177 ( P = 0.0128), p-ERK1/2 0.483 ± 0.135 ( P = 0.0027), vimentin 0.470 ± 0.160 ( P = 0.0047), N -cadherin 0.626 ± 0.140 ( P = 0.0008), E -cadherin 1.433 ± 0.223 ( P = 0.0284), C-JUN 0.413 ± 0.075 ( P = 0.0003), BCL-2 0.433 ± 0.102 ( P = 0.0007), and BAX 1.286 ± 0.175 ( P = 0.034) in miR-NC/miR-124-3p mimic-transfected HCCLM3 and (B) RAF 0.493 ± 0.158 ( P = 0.0054), MEK 0.603 ± 0.085 ( P = 0.0013), ERK1/2 0.513 ± 0.055 ( P = 0.0001), p-ERK1/2 0.666 ± 0.090 ( P = 0.0031), vimentin 0.626 ± 0.140 ( P = 0.0099), N -cadherin 0.476 ± 0.092 ( P = 0.0006), E -cadherin 1.526 ± 0.215 ( P = 0.0133) C-JUN 0.526 ± 0.070 ( P = 0.0003), BCL-2 0.696 ± 0.035 ( P = 0.0001), and BAX 1.863 ± 0.273 ( P = 0.0054) in Huh7. GAPDH was taken as the internal reference. The data was presented as mean ± SD of triplicate experiments for each protein. Unpaired Student t -test analysis was done for statistical significance. ∗ , ∗∗ , and ∗∗∗ refer to P -values below 0.05, 0.01, and 0.001.
Article Snippet: The primary antibodies with their dilution factors include CRKL (1:2000, Cat# GTX107677, United States), RAF (1:500, Thermo Scientific Cat# PA5-37713, USA Invitrogen), p-MEK1/2 (1:500, Cat# ABP50356 pSer218/222, Abbkine, China), ERK1/2 (1:1000, Cell Signaling Technology Cat# 9102, United States), p-ERK1/2 (1:500, Cell Signaling Technology Cat# 9106), C-JUN (1:500, Cat# ABP57278, Abbkine, China), BCL-2 (1:1000, Proteintech Cat# 12789-1-AP), BAX (1:1000, Proteintech Cat# 50599-2-Ig), vimentin (1:1000, Proteintech Cat# 10366-1-AP), N -cadherin (1:1000, Proteintech Cat# 22018-1-AP), E -cadherin (1:1000, Proteintech Cat# 20874-1-AP), ACTB (1:4000, Proteintech Cat# 66009-1-Ig),
Techniques: Expressing, Transfection